Effect of innovative ultrasonic frequency excitation modes on rice protein: Enzymolysis and structure

• DOI: 10.1016/j.lwt.2021.112435
• Publication Date: 2021-09-08T12:27:07Z
• Authors: Yanhua Ding; Yaoyao Wang; Wenjuan Qu; Xiaofeng Ren; Feng Lu; Weijie Tian; Janet Quaisie; S.M. Roknul Azam; Haile Ma
• Keywords: Random coil

Fixed frequency ultrasound (FFU), cyclic-sweeping (CSFU), and random-sweeping (RSFU) with the identical central were constructed, their effects on enzymatic hydrolysis structural characteristics of rice protein (RP) studied. FFU RSFU enhanced degree (DH) angiotensin-I converting enzyme (ACE) inhibitory activity RP hydrolysate significantly. CSFU increased ACE but had no significant effect DH. The molecular weight distribution results showed that promoted conversion to low peptides (1000–2000 200–1000 Da). Meanwhile, FFU, CSFU, pretreatment improved surface hydrophobicity index H0 from 397.53 464.23, 436.37, 477.6, respectively. Circular dichroism spectra indicated three ultrasonic modes decreased α-helix random coil content. Ultraviolet–visible atomic force microscopy caused exposure hydrophobic groups reduced particle size, Therefore, innovative modified its hydrolysate.