Optimization of the biotechnological process using Rhodotorula mucilaginosa and acerola (Malpighia emarginata L.) seeds for the production of bioactive compounds

• DOI: 10.1016/j.lwt.2022.113190
• Publication Date: 2022-02-22T10:31:53Z
• Authors: Nayjara Carvalho ...; Juliete Pedreira ...; Augusto de Souza ...; Paula Ferreira Ba...; Cecilia Morais Sa...; Murugan Rajan; Maria Terezinha S...; Narendra Narain
• Keywords: 0301 basic medicine; 03 medical and health sciences

The objective of the research was to optimize fermentation process by using acerola seeds with R. mucilaginosa obtain bioactive compounds. A Central Composite Rotational Design (CCRD) applied, independent variables, pH, yeast extract, residue and glucose, resulting in maximum values total carotenoids (1.50 mg/g) soluble phenolics (327 mg GAE/g) biomass, relation fermented broth, 1.12 mg/L 258 mg/L, respectively. UPHLC-DAD analysis identified gallic protocatechuic acids, hesperidin pyrocatechol. In 96 h fermentation, medium contained a higher concentration acid (0.50 β-carotene (20.9 ± 0.0 mg/g). Among organic acids HPLC-DAD, oxalic showed highest (83.6–90.3 mg/100 mL). an excellent substrate for production results indicate that submerged is strategy add value providing significant increase synthesis phenolic compounds important biological functions.