ARTP mutagenesis promotes selenium accumulation in Saccharomyces boulardii

• DOI: 10.1016/j.lwt.2022.113916
• Publication Date: 2022-09-02T15:33:24Z
• Authors: Xinling Nie; Yan Xing; Qingfei Li; Feng Gao; Shiyan Wang; Pei Liu; Xiangqian Li; Zhongbiao Tan; Pixiang Wang; Hao Shi
• Keywords: Strain (injury); Saccharomyces boulardii

Atmospheric and room temperature plasma (ARTP) is a rapid method for strain to obtain desirable mutants. In this study, Saccharomyces boulardii was used mutagenesis through ARTP the medium cultivation further optimized by Box–Behnken design experiments. The biomass of optimal mutant MSBV reached 42.8 g/L wet cell weight (WCW), total selenium (Se) 13.96 mg/g dry with addition 300 μg/mL sodium selenite at 0 h 48 cultivation. same conditions, wild type (WT) only 29.68 WCW, Se content 8.79 mg/g. addition, bound compounds accounted 76.84% protein, while WT 79.24%, which consistent energy dispersive spectroscopic analysis. Transmission electron microscopy images Se-enriched yeast showed that elemental Se0 had size approximately 50–200 nm nanoparticles were possibly coated protein. Therefore, it worth characterizing studying mechanism in future work. due its high accumulation content, shows great potential applications food, medical pharmaceutic industries.