Comparative study on the target sequences of thaumatin-like protein (TLP) and internal transcribed spacer (ITS) in the detection of corn component in edible oil by ladder-shape melting temperature isothermal amplification (LMTIA)

• DOI: 10.1016/j.lwt.2024.115903
• Publication Date: 2024-02-27T04:48:37Z
• Authors: Fugang Xiao; Yaoxuan Zhang; Menglin Gu; XiaoQi Xi; Yongqing Zhang; Juntao Sun; Quanzeng Wei; Bochun Hu; Guozhi Zhang; Deguo Wang
• Keywords: Thaumatin; Edible oil; Isothermal process

Single copy thaumatin-like protein (TLP) genes and multiple internal transcribed spacer (ITS) from the nucleus of corn were selected as targets, designed screened primers. The impact target sequence selection on ladder-shape melting temperature isothermal amplification (LMTIA) detection components in edible oil aspects such specificity, sensitivity, stability studied. results showed that LMTIA system targeting TLP has an optimal reaction 58 °C can detect a minimum 100 pg/μL DNA, established with ITS higher 59 °C, DNA low 1 pg/μL. Both systems have good stable reactions, no false positive observed. Conclusion: is more suitable for oil.