Kidney resident macrophages have distinct subsets and multifunctional roles

• DOI: 10.1016/j.matbio.2024.02.002
• Publication Date: 2024-02-07T01:52:16Z
• Authors: Christine Chew; Oliver J Brand; Tomohiko Yamamura; Craig Lawless; Mychel Raony Paiv...; Leo Zeef; I-Hsuan Lin; Gareth Howell; Sylvia Lui; Franziska Lausecker; Christopher Jagger; Tovah N Shaw; Siddharth Krishnan; Flora A McClure; Hayley Bridgeman; Kelly Wemyss; Joanne E Konkel; Tracy Hussell; Rachel Lennon
• Keywords: Monocyte; CX3CR1

The kidney contains distinct glomerular and tubulointerstitial compartments with diverse cell types extracellular matrix components. role of immune cells in environment is crucial for dampening inflammation maintaining homeostasis. Macrophages are innate that influenced by their tissue microenvironment. However, the multifunctional macrophages remains unclear. Flow imaging cytometry were used to determine relative expression CD81 CX3CR1 (C-X3-C motif chemokine receptor 1) macrophages. Monocyte replenishment was assessed Cx3cr1CreER X R26-yfp-reporter shielded chimeric mice. Bulk RNA-sequencing mass spectrometry-based proteomics performed on isolated from wild type Col4a5−/− (Alport) RNAscope visualize transcripts macrophage purity bulk RNA CIBERSORTx analyses. In mice we identified three subsets using these showed dependence monocyte replenishment. addition function, enrichment biological processes associated matrix. Proteomics collagen IV laminins whilst other proteins including cathepsins, ANXA2 LAMP2 enriched A subset co-expressed transcripts. We positive Multiomic analysis demonstrated have functions underscore importance health disease.