Easy genotyping of complement C3 ‘slow’ and ‘fast’ allotypes by tetra-primer amplification refractory mutation system PCR

• DOI: 10.1016/j.mcp.2010.07.002
• Publication Date: 2010-07-23T08:52:38Z
• Authors: Benedetta Peruzzi; Martina Serra; Chiara Pescucci; Michela Sica; Sonia Lastraioli; Tommaso Rondelli; Simona Pedemonte; Antonio Maria Ris...; Maria De Angioletti; Patrizia Piccioli; Rosario Notaro
• Keywords: Primer (cosmetics); Tetra; Complement

Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.