Estimation of paracrine signaling using quantitative RT-PCR from multiple patchy lesion samples

• DOI: 10.1016/j.mcp.2012.08.002
• Publication Date: 2012-08-17T15:17:19Z
• Authors: Shuang Jiao; Haruo Hanawa; Tomoyasu Suzuki; Ying Hua Hu; Kaori Yoshida; Minako Ogura; Yukako Ohno; Masahiro Ito; Takeshi Kashimura; Hiroaki Obata; Makoto Kodama
• Keywords: Disease Models, Animal; Myocarditis; 03 medical and health sciences; 0302 clinical medicine; Reverse Transcriptase Polymerase Chain Reaction; Paracrine Communication; Animals; RNA, Messenger; Autoimmune Diseases; Interleukin-1; Rats

We investigated whether correlations between mRNA levels of cytokines versus other proteins from patchy lesion could estimate cytokine paracrine signaling in vivo. Experiments with rat experimental autoimmune myocarditis (EAM), a patchy myocarditis model, indicated IL-1 and other protein levels were correlated, indicating paracrine signaling pathways in vivo.