miR-410-5p promotes the development of diabetic cardiomyopathy by suppressing PIM1-induced anti-apoptosis

• DOI: 10.1016/j.mcp.2020.101558
• Publication Date: 2020-03-09T05:48:40Z
• Authors: Xiaofen Xia; Yan Liang; Weihong Zheng; Dongming Lin; Shuping Sun
• Keywords: 0301 basic medicine; Base Sequence; Diabetic Cardiomyopathies; Myocardium; Apoptosis; 3. Good health; Rats, Sprague-Dawley; MicroRNAs; 03 medical and health sciences; Glucose; Proto-Oncogene Proteins c-pim-1; Animals; Myocytes, Cardiac

Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myocardial tissue of a diabetes mellitus (DM) rat model. Further studies confirmed that inhibition of miR-410-5p reduced cell apoptosis by regulating the Bcl-2/Bax axis. Through bioinformatics analysis and luciferase reporter assays, we observed that miR-410-5p directly targets PIM1. Moreover, knockdown of miR-410-5p by antagomir-410-5p improved diabetes-induced cardiac function and myocardial tissue structure. In conclusion, our study demonstrated that miR-410-5p might be involved in the progression of DCM by targeting PIM1 and might be a promising therapeutic target for DCM in the future.