Integrative Proteomics and N-Glycoproteomics Analyses of Rheumatoid Arthritis Synovium Reveal Immune-Associated Glycopeptides

• DOI: 10.1016/j.mcpro.2023.100540
• Publication Date: 2023-04-04T01:26:03Z
• Authors: Zhiqiang Xu; Yi Liu; Siyu He; Rui Sun; Chenxi Zhu; Shuangqing Li; Shan Hai; Yubin Luo; Yi Zhao; Lunzhi Dai
• Keywords: Glycoproteomics; Pathogenesis

Rheumatoid arthritis (RA) is a typical autoimmune disease characterized by synovial inflammation, tissue hyperplasia, and destruction of bone cartilage. Protein glycosylation plays key roles in the pathogenesis RA but in-depth glycoproteomics analysis tissues still lacking. Here, using strategy to quantify intact N-glycopeptides, we identified 1260 N-glycopeptides from 481 N-glycosites on 334 glycoproteins synovium. Bioinformatics revealed that hyper-glycosylated proteins were closely linked immune responses. By DNASTAR software, 20 whose prototype peptides highly immunogenic. We next calculated enrichment scores nine types cells specific gene sets public single-cell transcriptomics data N-glycosylation levels at some sites, such as IGSF10_N2147, MOXD2P_N404, PTCH2_N812, significantly correlated with certain cell types. Furthermore, showed aberrant synovium was related increased expression enzymes. Collectively, this work presents, for first time, N-glycoproteome describes immune-associated glycosylation, providing novel insights into pathogenesis.