Whole transcriptome analyses and comparison reveal the metabolic differences between oxidative and glycolytic skeletal muscles of yak

• DOI: 10.1016/j.meatsci.2022.108948
• Publication Date: 2022-08-11T02:59:41Z
• Authors: Xiaoyun Wu; Xuelan Zhou; Min Chu; Xian Guo; Jie Pei; Lin Xiong; Xiaoming Ma; Pengjia Bao; Chunnian Liang; Ping Yan
• Keywords: Mammals; 0301 basic medicine; 2. Zero hunger; Gene Expression Profiling; Phosphatidylinositol 3-Kinases; Oxidative Stress; 03 medical and health sciences; Animals; Cattle; RNA, Messenger; Muscle, Skeletal; Transcriptome; Glycolysis

Mammalian skeletal muscle is composed of various muscle fibers that exhibit different physiological and metabolic features. Muscle fiber type composition has significant influences on the meat quality of livestock. In this study, we comprehensively analyzed the whole transcriptome profiles of the oxidative muscle biceps femoris (BF) and the glycolytic muscle obliquus externus abdominis (OEA) of yak. A total of 1436 mRNAs, 1172 lncRNAs, and 218 circRNAs were differentially expressed in the oxidative muscles compared with the glycolytic muscles. KEGG annotation showed that differentially expressed mRNAs regulated by lncRNA and circRNA were mainly involved in PPAR signaling pathway, citrate cycle (TCA cycle), and PI3K-Akt signaling pathway, which reflect the different metabolic properties between oxidative and glycolytic muscles. In addition, regulatory networks associated with muscle fiber type conversion and mitochondria energy metabolism in muscles were constructed. Our study provides new evidence for a better understanding of the molecular mechanisms underlying skeletal muscle fiber determination and meat quality traits of yak.