Culturing clinical Methanobrevibacter smithii using GG medium in a minimal anaerobe atmosphere.

• DOI: 10.1016/j.mimet.2023.106704
• Publication Date: 2023-03-11T01:41:46Z
• Authors: Virginie Pilliol; Cheick Oumar Guindo; Elodie Terrer; Gérard Aboudharam; Michel Drancourt; Ghiles Grine
• Keywords: Methanogen; Isolation

Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane is, therefore, of paramount importance for equilibrium microbiota. The isolation by culture M. has routinely relied upon hydrogen‑carbon dioxide-enriched, oxygen-deprived atmospheres. In this study, we developed a medium referred to as "GG", which allowed growth in an atmosphere, with no supply either or carbon dioxide, making it easier detect clinical microbiology laboratories.