A Tethering Mechanism Controls the Processivity and Kinetochore-Microtubule Plus-End Enrichment of the Kinesin-8 Kif18A

• DOI: 10.1016/j.molcel.2011.07.022
• Publication Date: 2011-09-03T01:16:46Z
• Authors: Jason Stumpff; Yaqing Du; Chauca A. English; Zoltan Maliga; Michael Wagenbach; Charles L. Asbury; Linda Wordeman; Ryoma Ohi
• Keywords: Processivity; Kinesin; Tethering

Metaphase chromosome positioning depends on Kif18A, a kinesin-8 that accumulates at and suppresses the dynamics of K-MT plus ends. By engineering Kif18A mutants suppress MT but fail to concentrate ends, we identify mechanism allows accumulate ends level required movements. Enrichment its C-terminal tail domain, while ability growth is conferred by N-terminal motor domain. The contains second MT-binding domain diffuses along lattice, suggesting it tethers track. Consistently, enhances processivity crucial for heightened thus promotes concentration where their control