Metazoan Nuclear Pores Provide a Scaffold for Poised Genes and Mediate Induced Enhancer-Promoter Contacts

• DOI: 10.1016/j.molcel.2017.02.020
• Publication Date: 2017-03-30T16:02:06Z
• Authors: Pau Pascual-Garcia; Brian Debo; Jennifer R. Aleman; Jessica A. Talamas; Yemin Lan; Nha H. Nguyen; Kyoung J. Won; Maya Capelson
• Keywords: Transcriptional Activation; Ecdysone; 0303 health sciences; Binding Sites; Genotype; Transcription, Genetic; Transfection; Chromatin; Cell Line; Animals, Genetically Modified; Nuclear Pore Complex Proteins; 03 medical and health sciences; Drosophila melanogaster; Enhancer Elements, Genetic; Phenotype; Mutation; Animals; Drosophila Proteins; Insulator Elements; RNA Interference; Promoter Regions, Genetic; Protein Binding

Nuclear pore complex components (Nups) have been implicated in transcriptional regulation, yet what regulatory steps are controlled by metazoan Nups remains unclear. We identified the presence of multiple Nups at promoters, enhancers, and insulators in the Drosophila genome. In line with this binding, we uncovered a functional role for Nup98 in mediating enhancer-promoter looping at ecdysone-inducible genes. These genes were found to be stably associated with nuclear pores before and after activation. Although changing levels of Nup98 disrupted enhancer-promoter contacts, it did not affect ongoing transcription but instead compromised subsequent transcriptional activation or transcriptional memory. In support of the enhancer-looping role, we found Nup98 to gain and retain physical interactions with architectural proteins upon stimulation with ecdysone. Together, our data identify Nups as a class of architectural proteins for enhancers and supports a model in which animal genomes use the nuclear pore as an organizing scaffold for inducible poised genes.