A Role for Chromatin Remodeling in Cohesin Loading onto Chromosomes

• DOI: 10.1016/j.molcel.2019.02.027
• Publication Date: 2019-03-25T14:41:53Z
• Authors: Sofía Muñoz; Masashi Minamino; Corella S. Casas-...; Harshil Patel; Frank Uhlmann
• Keywords: Model organisms; 0301 basic medicine; Saccharomyces cerevisiae Proteins; 24 Ciencias de la Vida; DNA Repair; Transcription, Genetic; Chromosomal Proteins, Non-Histone; cohesin; nucleosomas; Cell Cycle Proteins; Saccharomyces cerevisiae; Biochemistry & Proteomics; Article; Chromosomes; Chromatin remodeling; 03 medical and health sciences; Scc2-Scc4; reparación del ADN; Chromosome Segregation; Sister chromatid cohesion; intercambio de cromátides hermanas; RSC; Cohesins; Computational & Systems Biology; Chemical Biology & High Throughput; chromosome condensation; 0303 health sciences; phosphorylation; proteínas de unión al ADN; Genome Integrity & Repair; Tumour Biology; Chromatin Assembly and Disassembly; Cohesin loader; segregación cromosómica; Nucleosomes; DNA-Binding Proteins; ensamblaje y desensamblaje de la cromatina; cromosomas; proteínas de Saccharomyces cerevisiae; Cell Cycle & Chromosomes; cell cycle; proteínas del ciclo celular; Sister Chromatid Exchange; Genetics & Genomics

Cohesin is a conserved, ring-shaped protein complex that topologically embraces DNA. Its central role in genome organization includes functions sister chromatid cohesion, DNA repair, and transcriptional regulation. loading onto chromosomes requires the Scc2-Scc4 cohesin loader, whose presence on chromatin budding yeast depends RSC remodeling complex. Here we reveal dual of loading. acts as receptor recruits by direct interaction independent remodeling. In addition, required to generate nucleosome-free region substrate for An engineered module can be created fusing Scc2 C terminus or other remodelers, but not unrelated binding proteins. These observations demonstrate importance provide insight into how accesses during its varied chromosomal activities.