c-di-GMP Arms an Anti-σ to Control Progression of Multicellular Differentiation in Streptomyces

• DOI: 10.1016/j.molcel.2019.11.006
• Publication Date: 2019-12-03T15:47:42Z
• Authors: Kelley A. Gallagher; Maria A. Schumacher; Matthew J. Bush; Maureen J. Bibb; Govind Chandra; Neil A. Holmes; Wenjie Zeng; Max Henderson; Hengshan Zhang; Kim C. Findlay; Richard G. Brennan; Mark J. Buttner
• Keywords: Multicellular organism

Streptomyces are our primary source of antibiotics, produced concomitantly with the transition from vegetative growth to sporulation in a complex developmental life cycle. We previously showed that signaling molecule c-di-GMP binds BldD, master repressor, control initiation development. Here we demonstrate also intervenes later development differentiation reproductive hyphae into spores by arming novel anti-σ (RsiG) bind and sequester sporulation-specific σ factor (σWhiG). present structure RsiG-(c-di-GMP)2-σWhiG complex, revealing an unusual, partially intercalated dimer bound at RsiG-σWhiG interface. RsiG absence σWhiG, employing E(X)3S(X)2R(X)3Q(X)3D motif repeated on each helix coiled coil. Further studies is essential for inhibit σWhiG. These findings reveal newly described mechanism σ-anti-σ formation establish as central integrator