Centromere/kinetochore is assembled through CENP-C oligomerization

• DOI: 10.1016/j.molcel.2023.05.023
• Publication Date: 2023-06-08T14:50:22Z
• Authors: Masatoshi Hara; Mariko Ariyoshi; Tomoki Sano; Ryu-Suke Nozawa; Soya Shinkai; Shuichi Onami; Isabelle Jansen; Toru Hirota; Tatsuo Fukagawa
• Keywords: centromere; Chromosomal Proteins, Non-Histone; Centromere; CCAN; chromosome segregation; kinetochore assembly; Humans; Kinetochores; Chromatin; Centromere Protein A; CENP-C; oligomerization

Kinetochore is an essential protein complex required for accurate chromosome segregation. The constitutive centromere-associated network (CCAN), a subcomplex of the kinetochore, associates with centromeric chromatin and provides platform kinetochore assembly. CCAN CENP-C thought to be central hub centromere/kinetochore organization. However, role in assembly needs elucidated. Here, we demonstrate that both CCAN-binding domain C-terminal region includes Cupin are necessary sufficient chicken function. Structural biochemical analyses reveal self-oligomerization domains human CENP-C. We find oligomerization vital function, localization CCAN, These results suggest facilitates through its oligomerization.