The Tartary Buckwheat Genome Provides Insights into Rutin Biosynthesis and Abiotic Stress Tolerance

• DOI: 10.1016/j.molp.2017.08.013
• Publication Date: 2017-09-01T17:15:14Z
• Authors: Lijun Zhang; Xiuxiu Li; Bin Ma; Qiang Gao; Huilong Du; Yuanhuai Han; Yan Li; Yinghao Cao; Ming Qi; Yaxin Zhu; Hongwei Lu; Mingchuan Ma; Longlong Liu; Jianping Zhou; Chenghu Nan; Yongjun Qin; Jun Wang; Lin Cui; Huimin Liu; Chengzhi Liang; Zhijun Qiao
• Keywords: Fosmid; Sequence assembly

Tartary buckwheat (Fagopyrum tataricum) is an important pseudocereal crop that strongly adapted to growth in adverse environments. Its gluten-free grain contains complete proteins with a well-balanced composition of essential amino acids and rich source beneficial phytochemicals provide significant health benefits. Here, we report high-quality, chromosome-scale genome sequence 489.3 Mb assembled by combining whole-genome shotgun sequencing both Illumina short reads single-molecule real-time long reads, tags large DNA insert fosmid library, Hi-C data, BioNano maps. We annotated 33 366 high-confidence protein-coding genes based on expression evidence. Comparisons the intra-genome sugar beet revealed independent duplication occurred lineage after they diverged from common ancestor, which was not shared rosids or asterids. The reference facilitated identification many new predicted be involved rutin biosynthesis regulation, aluminum stress resistance, drought cold responses. Our data suggest buckwheat's ability tolerate high levels abiotic attributed expansion several gene families signal transduction, membrane transport. availability these genomic resources will facilitate discovery agronomically nutritionally genetic improvement buckwheat.