Curcumin selectively induces colon cancer cell apoptosis and S cell cycle arrest by regulates Rb/E2F/p53 pathway

• DOI: 10.1016/j.molstruc.2022.133180
• Publication Date: 2022-04-27T09:32:34Z
• Authors: Peihua Li; Shiming Pu; Chao Lin; Liu He; Hongxia Zhao; Cheng Yang; Ziqi Guo; Shisan Xu; Zuping Zhou
• Keywords: Viability assay

Curcumin is a natural active product extracted from turmeric, has been reported to have anticancer properties against various tumors. However, the effect of curcumin on colon cancer remains still unclear. Our aimed investigate anti-colon in vitro, and determine molecular mechanisms underlying these effects. Viability assays including CCK8 LDH were used measure cell proliferation. Flow cytometry was performed detect apoptosis cycle distribution. qRT-PCR Western blotting for measuring genes/protein expression/activation apoptotic pro-carcinogenic cellular signaling pathways. Finally, results confirmed using xenograft tumor model that comprised mice. In data showed preferentially and, dose, dependent way inhibited cells proliferation, but not toxic normal mucosa epithelial cells. induced through p53-mediated mechanism, pro-apoptotic proteins Bax upregulated, arrest at S phase by expression related proteins, phosphorylation Rb pathway E2F family transcription factors. mouse suppressive growth vivo. may be potential strategy treatment cancer.