Molecularly imprinted polymer based on MWCNT-QDs as fluorescent biomimetic sensor for specific recognition of target protein

• DOI: 10.1016/j.msec.2014.12.032
• Publication Date: 2014-12-09T20:05:26Z
• Authors: Zhaoqiang Ding; S.W. Annie Bligh; Lei Tao; Jing Quan; Huali Nie; Limin Zhu; Xiao Gong
• Keywords: Nanotubes, Carbon; Serum Albumin, Bovine; 02 engineering and technology; Sensitivity and Specificity; Hemoglobins; Spectrometry, Fluorescence; Biomimetic Materials; Quantum Dots; Animals; Cattle; Muramidase; 0210 nano-technology

A novel molecularly imprinted optosensing material based on multi-walled carbon nanotube-quantum dots (MWCNT-QDs) has been designed and synthesized for its high selectivity, sensitivity and specificity in the recognition of a target protein bovine serum albumin (BSA). Molecularly imprinted polymer coated MWCNT-QDs using BSA as the template (BMIP-coated MWCNT-QDs) exhibits a fast mass-transfer speed with a response time of 25min. It is found that the BSA as a target protein can significantly quench the luminescence of BMIP-coated MWCNT-QDs in a concentration-dependent manner that is best described by a Stern-Volmer equation. The KSV for BSA is much higher than bovine hemoglobin and lysozyme, implying a highly selective recognition of the BMIP-coated MWCNT-QDs to BSA. Under optimal conditions, the relative fluorescence intensity of BMIP-coated MWCNT-QDs decreases linearly with the increasing target protein BSA in the concentration range of 5.0×10(-7)-35.0×10(-7)M with a detection limit of 80nM.