Multifunctional soft hybrid bio-platforms based on nano-silver and natural compounds

• DOI: 10.1016/j.msec.2016.07.077
• Publication Date: 2016-07-31T15:15:29Z
• Authors: M.E. Barbinta-Pat...; N. Badea; C. Pirvu; M. Bacalum; C. Ungureanu; P.L. Nadejde; C. Ion; I. Rau
• Keywords: Chlorophyll; Biological Products; Silver; Cell Death; Cell Survival; Chlorophyll A; Metal Nanoparticles; Microbial Sensitivity Tests; Microscopy, Atomic Force; Hemolysis; 01 natural sciences; Antioxidants; Anti-Bacterial Agents; 0104 chemical sciences; 3. Good health; Mice; Folic Acid; Escherichia coli; Animals; Humans; Particle Size; Cell Shape; HT29 Cells

Novel nanohybrids consisting of nano-Ag, chitosan, lipids and phyto-compounds (chlorophyll a and curcumin) have been achieved through a simple bottom-up strategy, resulting in stable (ZP=-30.9mV) and spherical-shaped nano-entities with size <200nm (estimated by AFM analysis and DLS measurements). The formation of these biohybrids was monitored by absorption and emission spectroscopy, exploiting the spectral fingerprint of chlorophyll a. The bio-performances of these hybrid materials such as: high antioxidant activity (96.63%), strong biocidal properties against Escherichia coli ATCC 8738 (exhibiting an inhibition zone diameter of 32mm), hemocompatibility, in vitro cytotoxicity against HT-29 cancer cells and no toxicity to normal cells (in the biohybrid concentration range of 5.7-17%), make them promising candidates in bio-applications (antimicrobial and antioxidant coating, cancer treatment).