Fabrication of hydrogel based nanocomposite scaffold containing bioactive glass nanoparticles for myocardial tissue engineering

• DOI: 10.1016/j.msec.2016.08.012
• Publication Date: 2016-08-06T07:20:13Z
• Authors: Zahra Barabadi; Mahmoud Azami; Esmaeel Sharifi; Roya Karimi; Nasrin Lotfibakhs...; Reza Roozafzoon; Mohammad Taghi Jo...; Jafar Ai
• Keywords: 0301 basic medicine; Calorimetry, Differential Scanning; Neovascularization, Physiologic; Cell Differentiation; Heart; Immunohistochemistry; Hydrogel, Polyethylene Glycol Dimethacrylate; Nanocomposites; 3. Good health; Endometrium; 03 medical and health sciences; Spectroscopy, Fourier Transform Infrared; Microscopy, Electron, Scanning; Humans; Nanoparticles; Regeneration; Cell Lineage; Female; Myocytes, Cardiac; Glass; Stromal Cells; Porosity; Cells, Cultured

Selecting suitable cell sources and angiogenesis induction are two important issues in myocardial tissue engineering. Human endometrial stromal cells (EnSCs) have been introduced as an abundant and easily available resource in regenerative medicine. Bioactive glass is an agent that induces angiogenesis and has been studied in some experiments. The aim of this study was to investigate in vitro differentiation capacity of endometrial stem cells into cardiomyocyte lineage and to evaluate capability of bioactive glass nanoparticles toward EnSCs differentiation into endothelial lineage and angiogenesis on hydrogel scaffold. Our findings suggests that endometrial stem cells could be programmed into cardiomyocyte linage and considered a suitable cell source for myocardial regeneration. This experiment also revealed that inclusion of bioactive glass nanoparticles in hydrogel scaffold could improve angiogenesis through differentiating EnSCs toward endothelial lineage and increasing level of vascular endothelial growth factor secretion.