Novel fusion peptides deliver exosomes to modify injectable thermo-sensitive hydrogels for bone regeneration

• DOI: 10.1016/j.mtbio.2021.100195
• Publication Date: 2021-12-27T17:44:44Z
• Authors: Shiqing Ma; Jinzhe Wu; Han Hu; Yuzhu Mu; Lei Zhang; Yifan Zhao; Xiaowei Bian; Wei Jing; Pengfei Wei; Bo Zhao; Jiayin Deng; Zihao Liu
• Keywords: Regenerative Medicine

Injectable thermo-sensitive hydrogels composed of small intestinal submucosa (SIS) with exosomes derived from bone marrow mesenchymal stem cells (BMSCs) are desired for regeneration. However, poor mechanical properties limit the clinical application SIS hydrogels. Herein, incorporated 3-(3,4-dihydroxyphenyl) propionic acid (CA) assessed. The results show that improved. In addition, retention and stability over time at defect site also challenges. Fusion peptides designed by connecting collagen-binding domines (CBDs) collagen type I/III exosomal capture CP05 (CRHSQMTVTSRL) directly or via rigid linkers (EAAAK). vitro experiments demonstrate fusion contribute to promoting positive effect on osteogenic differentiation BMSCs. Meanwhile, combining in treatment rat skull models reveal could enhance exosomes, thereby strengthen therapeutic defects. Therefore, CA modified appear be a promising strategy regenerative medicine.