A lysosomes and mitochondria dual-targeting AIE-active NIR photosensitizer: Constructing amphiphilic structure for enhanced antitumor activity and two-photon imaging

• DOI: 10.1016/j.mtbio.2023.100721
• Publication Date: 2023-07-06T01:36:22Z
• Authors: Shaozhen Wang; Yunhui Liao; Zhaoji Wu; Yihong Peng; Yuchen Liu; Yinghua Chen; Longquan Shao; Zhijie Zeng; Yanshan Liu
• Keywords: Biocompatibility; Organelle; Phototoxicity

Development of lysosomes and mitochondria dual-targeting photosensitizer with the virtues near-infrared (NIR) emission, highly efficient reactive oxygen generation, good phototoxicity biocompatibility is desirable in field imaging-guided photodynamic therapy (PDT) for cancer. Herein, a new positively charged amphiphilic organic compound (2-(2-(5-(7-(4-(diphenylamino)phenyl)benzo[c] [1,2,5]thiadiazol-4-yl)thiophen-2-yl)vinyl)-3-methylbenzo[d]thiazol-3-ium iodide) (ADB) based on D-A-π-A structure designed comprehensively investigated. ADB demonstrates special dual-organelles targeting, bright NIR aggregation-induced emission (AIE) at 736 nm, high singlet (1O2) quantum yield (0.442), as well photostability. In addition, can act two-photon imaging agent elaborate observation living cells blood vessel networks tissues. Upon light irradiation, obvious decrease mitochondrial membrane potential (MMP), abnormal morphology, phagocytotic vesicles lysosomal disruption are observed, which further induce cell apoptosis resulting enhanced antitumor activity cancer treatment. vivo experiments reveal that inhibit tumor growth efficiently upon exposure. These findings demonstrate this targeted has great clinical therapy, work provides avenue development multi-organelles photosensitizers