Increased concentrations of P2X7R in oligodendrocyte derived extracellular vesicles of Multiple sclerosis patients

• DOI: 10.1016/j.nbd.2024.106601
• Publication Date: 2024-07-11T00:18:33Z
• Authors: Cristina Agliardi; Franca Rosa Guerini; Milena Zanzottera; Elisabetta Bolognesi; Domenico Caputo; Marco Rovaris; Mario Clerici
• Keywords: Pathogenesis; Myelin oligodendrocyte glycoprotein

Activation of the purinergic receptor P2X7 (P2X7R) is believed to be deleterious in autoimmune diseases and it was hypothesized play a role pathogenesis MS. P2X7R an ATP-gated non-selective cationic channel; its activation can driven by high concentrations ATP leads generation large, cytolytic conductance pores. also result apoptosis as consequence caspase cascade via P2X7R-dependent stimulation NLRP3 inflammasome. We measured oligodendrocyte derived extracellular vesicles (ODEVs) MS patients healthy subjects. Sixty-eight (50 relapsing-remitting, RR-MS, 18 primary progressive, PP-MS) 57 controls (HC) were enrolled. ODEVs enriched from serum double step immunoaffinity method using anti OMGp (oligodendrocyte myelin glycoprotein) antibody. concentration lysates ELISA. One-way Anova test showed that significantly higher PP-MS (mean: 1742.89 pg/mL) compared both RR-MS 1277.33 (p < 0.001) HC 879.79 0.001). Comparison between statistically significant Pearson's correlations P2RX7 positively correlated with EDSS = 0.002, r 0.38, 0.15-0.57 95% CI) MSSS 0.004, 0.34, 0.12-0.54 scores, considering together (PP-MS + RR-MS) disease duration group 0.02, 0.53, 0.09-0.80 CI). Results suggest ODEVs-associated levels could biomarker for