Ventromedial hypothalamic lesions change the expression of neuron-related genes and immune-related genes in rat liver

• DOI: 10.1016/j.neulet.2009.03.062
• Publication Date: 2009-03-26T05:19:29Z
• Authors: Takayoshi Kiba; Yuri Kintaka; Yoko Suzuki; Eiko Nakata; Yasuhito Ishigaki; Shuji Inoue
• Keywords: Neurons; 0301 basic medicine; Neuroimmunomodulation; Gene Expression Profiling; Hypothalamus; Suppressor of Cytokine Signaling Proteins; Polymerase Chain Reaction; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Gene Expression Regulation; Liver; Basic Helix-Loop-Helix Transcription Factors; Animals; Female; Sulfotransferases; Oligonucleotide Array Sequence Analysis

There are no reports that hypothalamus can directly affect the expression of neuron-related genes and immune-related genes in liver. We identified genes of which expression profiles showed significant modulation in rat liver after ventromedial hypothalamic (VMH) lesions. Total RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH lesioned rats were investigated using DNA microarray analysis. The result revealed that VMH lesions regulated the genes that were involved in functions related to neuronal development and immunofunction in the liver. Real-time PCR also confirmed that gene expression of SULT4A1 was upregulated, but expression of ACSL1 and CISH were downregulated at day 3 after VMH lesions. VMH lesions may change the expression of neuron-related genes and immune-related genes in rat liver.