GC–MS-based metabolomics identifies an amino acid signature of acute ischemic stroke

• DOI: 10.1016/j.neulet.2017.01.039
• Publication Date: 2017-01-19T14:17:18Z
• Authors: Dian Wang; Jing Kong; Jiayan Wu; Xingxing Wang; Minchao Lai
• Keywords: Metabolome; Hypoxanthine

Ischemic stroke is a serious public health problem worldwide. Here, we characterized the metabolite features of acute ischemic (AIS) using gas chromatography-mass spectrometry-based metabolomics. Forty AIS patients and 29 sex age-matched controls were recruited. The serum metabolic profiles significantly different between two groups. Compared to controls, had elevated levels lactate, carbonate glutamate (P<0.01), lowered alanine, citrate, glycine, isoleucine, leucine, serine, tyrosine, methionine, tryptophan, erythronic acid, urea, purine, hypoxanthine, proline in (P<0.05). Amino acids are most important disturbed metabolites. Most dysregulated metabolites closely correlated with each other several biochemical indices. differential reflect pathophysiological processes inflammation, energy deficit, oxidative stress, neurotoxicity, neuro excitation injury. Tyrosine, tryptophan screened as panel potential biomarkers AIS, jointly enabling high precision (91.7%) diagnose by classification regression tree (CRT). Collectively, discovered marked perturbation metabolome serum, mainly associated amino acid-related metabolism. may be considered AIS.