Functional magnetic resonance imaging of sound pressure level encoding in the rat central auditory system

• DOI: 10.1016/j.neuroimage.2012.09.069
• Publication Date: 2012-10-03T20:46:29Z
• Authors: Jevin W. Zhang; Condon Lau; Joe S. Cheng; Kyle K. Xing; Iris Y. Zhou; Matthew M. Cheung; Ed X. Wu
• Keywords: Male; 0301 basic medicine; Inferior colliculus; Rats, Sprague-Dawley; 03 medical and health sciences; Fmri; Sound pressure level; Image Processing, Computer-Assisted; Animals; Sound Pressure Level; Lateral lemniscus; Inferior Colliculus; Bold; Auditory Cortex; Brain Mapping; Auditory cortex; Hemodynamics; 621; Brain; Lateral Lemniscus; Medial geniculate body; Magnetic Resonance Imaging; Rats; Medial Geniculate Body; Sound; FMRI; Auditory Perception; Rat; BOLD

Intensity is an important physical property of a sound wave and is customarily reported as sound pressure level (SPL). Invasive techniques such as electrical recordings, which typically examine one brain region at a time, have been used to study neuronal encoding of SPL throughout the central auditory system. Non-invasive functional magnetic resonance imaging (fMRI) with large field of view can simultaneously examine multiple auditory structures. We applied fMRI to measure the hemodynamic responses in the rat brain during sound stimulation at seven SPLs over a 72 dB range. This study used a sparse temporal sampling paradigm to reduce the adverse effects of scanner noise. Hemodynamic responses were measured from the central nucleus of the inferior colliculus (CIC), external cortex of the inferior colliculus (ECIC), lateral lemniscus (LL), medial geniculate body (MGB), and auditory cortex (AC). BOLD signal changes generally increase significantly (p<0.001) with SPL and the dependence is monotonic in CIC, ECIC, and LL. The ECIC has higher BOLD signal change than CIC and LL at high SPLs. The difference between BOLD signal changes at high and low SPLs is less in the MGB and AC. This suggests that the SPL dependences of the LL and IC are different from those in the MGB and AC and the SPL dependence of the CIC is different from that of the ECIC. These observations are likely related to earlier observations that neurons with firing rates that increase monotonically with SPL are dominant in the CIC, ECIC, and LL while non-monotonic neurons are dominant in the MGB and AC. Further, the IC's SPL dependence measured in this study is very similar to that measured in our earlier study using the continuous imaging method. Therefore, sparse temporal sampling may not be a prerequisite in auditory fMRI studies of the IC.