Aryl hydrocarbon receptor-mediated apoptosis of neuronal cells: A possible interaction with estrogen receptor signaling

• DOI: 10.1016/j.neuroscience.2008.10.045
• Publication Date: 2008-11-01T08:46:55Z
• Authors: M. Kajta; A.K. Wójtowicz; M. Maćkowiak; W. Lasoń
• Keywords: Neurons; Selective Estrogen Receptor Modulators; 0301 basic medicine; Time Factors; Dose-Response Relationship, Drug; L-Lactate Dehydrogenase; Caspase 3; Estrogen Antagonists; Apoptosis; Neocortex; Embryo, Mammalian; Fluoresceins; Hippocampus; Mice; Tamoxifen; 03 medical and health sciences; Receptors, Aryl Hydrocarbon; Receptors, Estrogen; Animals; Enzyme Inhibitors; Cells, Cultured; Signal Transduction

Activation of aryl hydrocarbon receptors (AhRs) induces neuronal damage, but the mechanism by which this occurs is largely unknown. This study evaluated the effects of an AhR agonist, beta-naphthoflavone, on apoptotic pathways in mouse primary neuronal cell cultures. beta-Naphthoflavone (0.1-100 micronhanced caspase-3 activity and lactate dehydrogenase (LDH) release in neocortical and hippocampal cells. These data were supported at the cellular level with Hoechst 33342 and calcein AM staining. alpha-Naphthoflavone inhibited the action of beta-naphthoflavone, thus confirming specific activation of AhRs. A high-affinity estrogen receptor (ER) antagonist, ICI 182,780, and a selective estrogen receptor modulator (SERM), tamoxifen, enhanced beta-naphthoflavone-mediated apoptosis. Another SERM, raloxifene, and an ERalpha antagonist, methyl-piperidino-pyrazole, did not affect beta-naphthoflavone-induced caspase-3 activity. However, they inhibited beta-naphthoflavone-induced LDH release at a late hour of treatment, thus suggesting delayed control of AhR-mediated neuronal cell death. The apoptotic effects of beta-naphthoflavone were accompanied by increased levels of AhRs, and these receptors colocalized with ERbeta as demonstrated by confocal microscopy. These data strongly support apoptotic effects of AhR activation in neocortical and hippocampal tissues. Moreover, this study provides evidence for direct interaction of the AhR-mediated apoptotic pathway with estrogen receptor signaling, which provides insight into new strategies to treat or prevent AhR-mediated neurotoxicity.