In-vitro Approaches to Investigate the Detrimental Effect of Light on Dopaminergic Neurons

• DOI: 10.1016/j.neuroscience.2024.01.009
• Publication Date: 2024-01-18T17:34:19Z
• Authors: Irene Fasciani; Francesco Petragnano; Federica Bono; Gabriella Aloisi; Veronica Mutti; Carla Pardini; Marco Carli; Marco Scarselli; Francesca Vaglini; Adriano Angelucci; Chiara Fiorentini; Luca Lozzi; Cristina Missale; Roberto Maggio; Mario Rossi
• Keywords: MN9D cells; Parkinson’s disease; dopamine; hiPSC; light; oxidative stress; Dopamine; Light; MN9D cells; Oxidative stress; Parkinson’s disease; hiPSC

Our recent study revealed that fluorescent lamp light can penetrate deep into the brain of mice and rats leading to development typical histological characteristics associated with Parkinson's disease such as loss dopamine neurons in substantia nigra. Monochromatic LED lights were thus used this work deepen our knowledge on effects major wavelength peaks mouse human dopaminergic cells. In particular, we exposed immortalized MN9D neuronal cells, primary cultures mesencephalic cells differentiated from induced pluripotent stem (hiPSC) different wavelengths. We found chronic exposure reduced overall undifferentiated cell number, most significant observed at wavelengths 485 nm 610 nm. Moreover, especially was able negatively impact survival derived hiPSC. Notably, which closely resemble mature phenotype, acutely for 3h nm, showed a clear increase ROS production cytotoxicity compared controls These increases even more pronounced by co-treatment oxidative agent H2O2. Collectively, these findings suggest specific wavelengths, particularly those capable penetrating brain, could potentially pose an environmental hazard relation disease.