Platelet dysfunction in a large-animal model of endotoxic shock; effects of inhaled nitric oxide and low-dose steroid

• DOI: 10.1016/j.niox.2020.12.008
• Publication Date: 2021-01-02T16:22:21Z
• Authors: Barbara Adamik; Claes Frostell; Urszula Paslawska; Barbara Dragan; Stanislaw Zielinski; Robert Paslawski; Adrian Janiszewski; Marzena Zielinska; Stanislaw Ryniak; Gustaf Ledin; Waldemar Gozdzik
• Keywords: Blood Platelets; 0301 basic medicine; Hydrocortisone; Platelet Aggregation; Swine; Vasodilator Agents; Nitric Oxide; Shock, Septic; 3. Good health; Endotoxins; 03 medical and health sciences; Administration, Inhalation; Animals; Pulmonary Wedge Pressure

The role of inhaled nitric oxide in the treatment shock remains controversial and further translational research is needed. Long-term observation studies using a model endotoxin-induced to assess effect on platelet aggregation have not yet been reported.The tests were carried out an animal two 10-h periods. During first 10 h, endotoxin was infused inhibition evaluated; following termination infusion, restoration assessed for h. A total 30 pigs used (NO group, N = 14; control, 16). In NO inhalation (30 ppm) started 3 h after infusion continued until end study. Treatment with selectively decreased pulmonary artery pressure at 4 (p 0.002) 8 0.05) experiment as compared control. Endotoxin significantly reduced aggregation, indicated by activity receptors: ASPI, ADP, collagen, TRAP during < 0.001). had no significant changes response receptor ristocetin stimulation. After stopping observed collagen TRAP, while ASPI ADP remained partially depressed. Inhaled did cause additional either or challenge.A profound reduction endotoxic shock. seen. induced intensified oxide, indicating there harmful aggregation.