Cerebral pCREB-dependent social behavioral adversities following a short-term exposure to obesogenic diets in young hamsters

• DOI: 10.1016/j.ntt.2022.107094
• Publication Date: 2022-05-02T15:18:33Z
• Authors: Merylin Zizza; Gilda Fazzari; Mariana Di Lorenzo; Raffaella Alò; Rosalinda Bruno; Vincenza Laforgia; Marcello Canonaco; Rosa Maria Facciolo
• Keywords: Cerebral Cortex; Male; 0301 basic medicine; 2. Zero hunger; Memory Disorders; Behavior, Animal; CREB activation; Dietary Sugars; Body Weight; Sociability; Abdominal Fat; Organ Size; Diet, High-Fat; Short-term westernized diet, Sociability, CREB activation , Limbic areas; Aggression; 03 medical and health sciences; Liver; Cricetinae; Short-term westernized diet; Animals; Limbic areas; Cyclic AMP Response Element-Binding Protein; Social Behavior; Short-term westernized diet, Sociability, CREB activation, Limbic areas

Excessive fat and sugar intake represents a risk towards the development of different pathologies, such as obesity, diabetes, sociability and memory deficits. Although the adolescence stage is a susceptible period for these and other risks, effects of energy-dense nutrients in such an age period have not been fully investigated. In the present study, neurobehavioral alterations following a 4-week exposure to either normal diet (ND) or high-fat diet (HFD) plus normal water (NW) or liquid sugar (LS) were evaluated in young hamsters. HFD + LS and ND + LS significantly reduced food intake and water consumption, which was, in the latter group, almost completely substituted by LS. All obesogenic diets accounted for increased abdominal fat and liver weight with respect to body weight (p < 0.05-0.001). Additionally, glucose levels notably increased (p < 0.0001) together with insulin and triglycerides in HFD + LS (p < 0.001) and ND + LS (p < 0.01) while cholesterol displayed only a moderate increase (p < 0.05) in HFD + NW and HFD + LS. Animals fed with HFD and/or LS exhibited impaired social memory plus increased winning percentages (0.05 < p < 0.01) during the tube test. Interestingly, these same treatments led to a down-regulation of phosphorylated cAMP Response-Element Binding Protein (pCREB) in HFD + NW (p < 0.0001) for all areas, but rather was upregulated (p < 0.05) in ND + LS of the amygdala. Overall, in view of a brief exposure to palatable foods interfering with normal metabolic and social memory activities, the downregulation of pCREB constitutes a key indicator of neurobehavioral deficits during obesogenic diets. Compensatory mechanisms may be also occurring in the amygdala that strongly regulates emotional states via connections with other limbic areas.