Design of a composite wound dressing: Combining an electrospun fleece with a free-standing multilayer film

• DOI: 10.1016/j.nxmate.2023.100060
• Publication Date: 2024-02-08T23:21:50Z
• Authors: Adrian Hautmann; Tobias Hedtke; Sonia Sislema-Muñoz; Juliana Martins-S...; Christian E.H. Sc...; Thomas Groth
• Keywords: Wound dressing; Hands free; Composite film

Chronic skin wounds place a heavy burden on patients and healthcare systems. To address this problem, we have developed novel composite material consisting of an electrospun fleece free-standing multilayer film that combines the wound healing benefits both materials. In detail combination spray coating electrospinning is used to create layer-by-layer top gelatin fleece, with final thickness about 1 mm. A partially crosslinked in formaldehyde vapor 30 pH-sensitive bonding bilayers oxidized hyaluronic acid (HA) chitosan, followed by 120 alginate chitosan are sprayed top. The resulting genipin. Uncrosslinked genipin composites compared unprocessed film. method produces stable composite, allows fast growth part und most importantly retains nano-topography side as confirmed electron microscopy, profilometry, nano-tomography dynamic mechanical analysis. test biocompatibility, cell proliferation experiments human dermal fibroblasts THP-1 derived macrophages performed, proliferative assays accompanied immunohistochemical staining pro/anti-inflammatory cytokine assay. shows no cytotoxicity biocompatible vitro. Furthermore, fibers act scaffold highly promote adhesion proliferation, while modular design film, crosslinking, tuning anti-inflammatory effect HA. Overall, seems be promising starting point for dressing.