Structural and antibacterial evaluation of copper, silver, and bimetallic silver/copper nanoalloys synthesized in chitosan biopolymer

• DOI: 10.1016/j.nxmate.2023.100071
• Publication Date: 2023-11-22T13:29:08Z
• Authors: Vanessa de Cassia...; Bruna Naemi Harada; Vitor Gonçalves V...; Ricardo Alexandre...; Suzan Pantaroto d...; Diogo Silva Pellosi
• Keywords: Bimetallic strip; Nanomaterials; Biopolymer; Silver nanoparticle

Silver nanoparticles (AgNP) have been used as an alternative to microbial resistance. Nevertheless, AgNP high cost and moderate toxicity humans limit their use. Copper (CuNP) emerge alternative, but fast oxidation hampers large-scale In this scenario, work propose the synthesis optimization of Ag, Cu, bimetallic Ag/Cu in a chitosan aqueous solution using factorial design statistics. UV-Vis spectroscopy, zetasizer, transmission electron microscopy (TEM) measurements demonstrated that optimized synthetic procedure formed highly stable monodisperse nanoparticles. X-ray diffraction (XRD) confirmed nanoparticle's crystalline structure indicated presence oxide state CuNP. addition, photoelectron spectroscopy (XPS) occurs at CuNP surface level. Considering Ag/CuNP, Rietveld refinement XRD diffractogram demonstrates formation unusual novel nanoalloy structure, which was possible due metallic ion chelation nitrogens before during nanoparticle growth. silver inhibited copper through electromigration mechanism. Comparing CuNP, AgNP, it reveal microbicide abilities against both Gram-positive Gram-negative bacteria, emphasizing is viable option its antimicrobial activity colloidal stability. Therefore, rational allowed NP matrix exhibited strong activity, indicating nanoalloys can act next-generation nanomaterials for pathogenic microorganism inactivation.