Calcified cartilage revealed in whole joint by X-ray phase contrast imaging

• DOI: 10.1016/j.ocarto.2021.100168
• Publication Date: 2021-04-26T05:59:48Z
• Authors: Ludovic Broche; Bertrand Favier; Hélène Rougé-Labriet; Sabine Drevet; Bernard Lardy; Emmanuel Brun; Benjamin Lemasson
• Keywords: X-Ray Phase-Contrast Imaging

X-ray Phase Contrast Imaging (PCI) is an emerging modality that will be in the next few years available a wider range of preclinical set-ups. In this study, we compare imaging technique with conventional modalities osteoarthritis mouse model. contrast was performed on 6 post-mortem, monoiodoacetate-induced knees and control knees. The mice were then imaged using magnetic resonance micro computed tomography. Examples surrogate markers are reported: local distances within articular space, cartilage surface roughness, calcified thickness, number, volume locations osteophytes. Thanks to PCI, can show 3D without agent by non-invasive technique. phase images reveal more details than techniques, especially at smaller scales, for instance higher number micro-calcifications detected (57, 314 329 MRI, micro-CT respectively). Calcified thickness measured significant difference (p ​< ​0.01) between (23.4 ​± ​17.2 ​μm) induced animal (46.9 ​19.0 ​μm). outperforms assessing different tissue types (soft hard). This new seems bring relevant following-up small models even low-grade osteoarthritis.