Cloning and expression analysis of HXK1 gene from Jatropha curcas L.

• DOI: 10.1016/j.ocsci.2020.05.004
• Publication Date: 2020-05-18T05:26:11Z
• Authors: Hu Xin; Lizhou Tang; Chao Liu; Yong Gao; Dongqin Dai; Huanhuan Chen; Yan Deng; Bin Tian; Haibo Wang
• Keywords: Jatropha curcas; Cloning (programming); Hexokinase

HXK (Hexokinase) gene family and the role of JcHXK1 in Jatropha curcas L. were explored. Totally 4 genes JcHXK1, JcHXK2, JcHXK3 JcHKL1 identified complete ORF was cloned. Functional domain, phylogenetic evolution low-temperature expression characteristics analyzed. Results showed that full-length cDNA 1497 bp, encoding 498 amino acids with molecular weight 53.81 ​kDa pI 5.03. Further evolutionary analysis demonstrated HXK1 protein clustered into 6 small branches 2 large branches. Sequence alignment contained several conserved glycine residues hydrophobic channels. Prokaryotic vector constructed 12% SDS-PAGE detection it highly expressed E. coli. These research expected to lay a foundation for further functional verification cold signal transduction mechanism