Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo

• DOI: 10.1016/j.omto.2021.09.009
• Publication Date: 2021-10-06T01:43:18Z
• Authors: Reshma Koolaparam...; Azeem Abdul Kalam; Joydeep Nag; Vishnu Sunil Jaik...; Umerali Kunnakkadan; Nisha Asok Kumar; Sreenath Muraleed...; Arumugam Rajavelu; John Bernet Johnson
• Keywords: Cytopathic effect; HeLa; Virotherapy

Chandipura virus (CHPV) is an emerging human pathogen of great clinical significance. In this study, we have investigated the susceptibility pattern both normal and cancer cell lines origin to wild-type (wt) CHPV in order explore possibility developing as oncolytic vector (OV). Marked cytopathic effect along with enhanced output was observed (HeLa, A549, U-138, PC-3, HepG2) comparison adult dermal fibroblast (HADF) cells. At MOI 0.1, were differentially susceptible CHPV, cells like HeLa U-138 having pronounced death, while PC-3 comparatively resistant. All used study except restricted infection varying degrees IFN-β pre-treatment supplementation interferon (IFN) could neither activate IFN signaling pathway Finally, tumor xenografts established non-obese diabetic severe combined immunodeficiency (NOD/SCID) mice showed significant delay growth CHPV-challenged animals. Thus, targeted at a very low dose replication offers rationale exploit future.