Label-free detection of inclusion body formation in E. coli with application of terahertz

• DOI: 10.1016/j.optcom.2024.130914
• Publication Date: 2024-07-30T15:52:10Z
• Authors: Hyerim Cho; Eui young Rho; Jisung Kwak; Hyun Seok Song; Minah Seo

Extensive production of recombinant protein is essential for use in various fields. Bacterial cells, especially the Escherichia coli (E. coli) expression system, are widely used proteins due to their advantages time and cost. However, formation inclusion bodies (IBs) caused by a significant issue. Therefore, precise tool detection IBs high demand. In this study, we demonstrated label-free rapid sensing platform E. using terahertz-time domain spectroscopy (THz-TDS) system terahertz metamaterials. The was based on local polarity difference between samples with induced (positive) non-induced (negative) overexpression. positive resulted decrease compared negative samples. This difference, strongly related intermolecular interactions, influenced THz absorption as regime involves weak vibrational modes. Experimental results showed that transmittance (ΔT) resonance frequency shift (Δf) were significantly different samples, confirming effectiveness our method. Specifically, exhibited ΔT value 26.57% Δf 0.12 THz, while 31.83% 0.14 THz. These highlight capability detect rapidly accurately.