Protective effects of MLIF analogs on cerebral ischemia-reperfusion injury in rats

• DOI: 10.1016/j.peptides.2011.03.005
• Publication Date: 2011-03-13T08:32:22Z
• Authors: Jia Yao; Ying Xu; Fang Ji; Chao Wang; Yun Zhang; Jingman Ni; Rui Wang
• Keywords: Male; Superoxide Dismutase; Tumor Necrosis Factor-alpha; Interleukin-1beta; Anti-Inflammatory Agents; Enzyme-Linked Immunosorbent Assay; Brain Ischemia; Rats; 3. Good health; Rats, Sprague-Dawley; 03 medical and health sciences; 0302 clinical medicine; Cell Movement; Malondialdehyde; Reperfusion Injury; Cell Adhesion; Animals; Oligopeptides

The monocyte locomotion inhibitory factor (MLIF) is an anti-inflammatory oligopeptide produced by Entamoeba histolytica. Among its different effects, it inhibits locomotion of human monocytes, hence its original name. The carboxyl-terminal end group Cys-Asn-Ser is the pharmacophore of anti-inflammatory peptide Met-Gln-Cys-Asn-Ser. In this study, the N-terminal of Cys-Asn-Ser was modified. With the aim to enhance the antioxidant ability and penetrability of Cys-Asn-Ser, we designed and synthesized two tetrapeptides Tyr-Cys-Asn-Ser and His-Cys-Asn-Ser. The neuroprotective effects of Tyr-Cys-Asn-Ser and His-Cys-Asn-Ser on focal ischemia reperfusion were investigated, and their pharmacological activities compared with Cys-Asn-Ser were studied. In order to study the mechanism of neuroprotective effect of these peptides, the level of oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) and pro-inflammatory factors interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and myeloperoxidase (MPO) were detected in brain tissue homogenate.