Shenxiong Yixin Decoction polysaccharides exert therapeutic effects against myocardial ischemia-reperfusion injury via intestinal exosomal microRNA-21 regulation

• DOI: 10.1016/j.phrs.2025.107725
• Publication Date: 2025-04-08T22:03:21Z
• Authors: De-Sheng Xu; Yan Wang; Zi-Rui Li; Ping-Li Mo; Ming-Tai Chen; Rui-Zhi Zhao; Zheng Fan; Wen-Jie Mo; Qi-Lu Li; Fang-Cao Pi; Qi-Di Sun; Juan-Min Li; Yun-Da Yao; Jian-Ping Chen; Yuan-Yuan Li; Jie-Nan Luan; Shang-Bin Zhang; Ying Xie; Hua Zhou
• Keywords: Decoction; Intestinal Ischemia

Background and Objective Myocardial ischemia-reperfusion injury (MIRI) presents a prevalent global clinical challenge without an optimal treatment strategy. Shenxiong Yixin Decoction exhibits promising cardioprotective effects in scenarios. Polysaccharides, particularly pivotal cardioprotection, act systemically through the intestine. Exosomes, miRNA-rich ones, are crucial MIRI pathophysiology. However, specific role of gut-derived exosomal miRNAs cardiovascular disease remains unclear. This study aimed to evaluate polysaccharides (SXYXP) on elucidate its mechanisms. In vitro vivo models were set up with H9c2 cells, C57BL/6 mice, Sprague-Dawley rats. Exosomes from IEC-6 cells plasma detected. Various techniques like MTT, TTC, H&E staining, echocardiography, flow cytometry, immunofluorescence, RT-qPCR, Western blot employed for pharmacodynamic mechanistic analyses. SXYXP can reduce myocardial infarction area, improve cardiac function, increase cell survival rate under MIRI. The significantly enhanced release enterocyte-derived exosomes elevated levels miR-21 within these exosomes. These SXYXP-modulated demonstrated significant protective effect against H/R cells. application inhibitors group negated exosomes, leading diminished cardiomyocyte proliferation, exacerbated oxidative stress, apoptosis. may protect by targeting intestinal cell-derived which transported heart blood restore level damaged myocardium, thereby inhibiting stress expression PDCD4.