Alkaloid chemophenetics and transcriptomics of the Nicotiana genus

• DOI: 10.1016/j.phytochem.2020.112424
• Publication Date: 2020-06-08T16:38:07Z
• Authors: Kacper Piotr Kami...; Lucien Bovet; Helene Laparra; Gerhard Lang; Damien De Palo; Nicolas Sierro; Simon Goepfert; Nikolai V. Ivanov
• Keywords: Nornicotine; Anabasine

In this study, we determined the pyridine alkaloid content (nicotine, nornicotine, anabasine, anatabine, cotinine, and myosmine) of 58 species 2 subspecies Nicotiana genus by ultra-high-performance liquid chromatography coupled with mass spectrometry. We observed clear correlation between Noctiflorae Suaveolentes sections their above average accumulation anabasine in genus. addition, results demonstrated presence not only trace amounts but quantifiable levels myosmine, an previously detected minute quantities, leaves roots 16 species. analysis gene expression from mRNA sequencing was performed for first time. Sequencing reads were mapped against annotated genes a tabacum reference genome values subsequently calculated. Hierarchical clustering biosynthesis pathway composition revealed patterns clearly segregating sections. Correlation phenotypes evident, including low putrescine methyltransferase all section or nicotine demethylase conversion rates to nornicotine majority Multiple additional correlations identified, which makes study important fundament toward future scientific exploration