SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy

• DOI: 10.1016/j.placenta.2023.04.009
• Publication Date: 2023-04-09T00:54:08Z
• Authors: Yuxuan Jiang; Xiaoping Yin; Qian Xu; Xiaoxiao Tang; Huajie Zhang; Xueshan Cao; Jing Lin; Yi Wang; Fei Yang; Naseer Ullah Khan; Liming Shen; Danqing Zhao
• Keywords: Cholestasis of pregnancy; Syncytiotrophoblasts; Placentation; Pathogenesis

Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought identify potential proteins placental tissue that may be involved pathogenesis ICP adverse fetal outcomes.The postpartum pregnant women with were chosen as case group (ICP group) (subdivided into mild (MICP severe (SICP group)), healthy control (CTR). hematoxylin-eosin (HE) staining was used observe histologic changes placenta. SWATH analysis combined liquid chromatography-tandem mass spectrometry (LC-MS) screen differentially expressed (DEPs) CTR groups, bioinformatics find out biological process these differential proteins.Proteomic studies showed there 126 DEPs from women. Most identified functionally related humoral immune response, cell response lipopolysaccharide, antioxidant activity heme metabolism. A subsequent examination placentas patients revealed 48 expressed. Through death domain receptors fibrinogen complexes, primarily regulate extrinsic apoptotic signaling pathways, blood coagulation, fibrin clot formation. expressions HBD, HPX, PDE3A, PRG4 down-regulated by Western blot analysis, which consistent proteomics.This preliminary helps us understand proteome patients, provides new insights pathophysiology ICP.