Production of ethanol from microwave-assisted alkali pretreated wheat straw

• DOI: 10.1016/j.procbio.2005.10.024
• Publication Date: 2005-11-29T08:49:43Z
• Authors: Shengdong Zhu; Yuanxin Wu; Ziniu Yu; Xuan Zhang; Cunwen Wang; Faquan Yu; Shiwei Jin
• Keywords: Enzymatic Hydrolysis

Abstract In order to evaluate the suitability of production of ethanol from the microwave-assisted alkali pretreated wheat straw, the simultaneous saccharification and fermentation (SSF) of the microwave-assisted and conventional alkali pretreated wheat straw to ethanol were optimized and compared using cellulase from Trichoderma reesei and Saccharomyces cerevisiae YC-097 cells. The SSF optima for the microwave-assisted alkali pretreated wheat straw were 100 g l−1 substrate, 40 °C, 15 mg [cellulase] g−1 [substrate], initial pH 5.3 and 72 h. Under the above optimum conditions, the ethanol concentration reached 34.3 g l−1 and the ethanol yield was 69.3%. The SSF optima for the conventional alkali pretreated wheat straw were 100 g l−1 substrate, 40 °C, 20 mg [cellulase] g−1 [substrate], initial pH 5.3 and 96 h. Under its optimum conditions, the ethanol concentration and the ethanol yield were 31.1 g l−1 and 64.8%, respectively. It was obvious that production of ethanol from microwave-assisted pretreated wheat straw had lower enzyme loading, shorter reaction time and could achieve higher ethanol concentration and yield than from the conventional alkali pretreated wheat straw. The microwave-assisted alkali pretreatment was an efficient pretreatment method of wheat straw for its ethanol production.