Easily handling penicillin G acylase magnetic cross-linked enzymes aggregates: Catalytic and morphological studies

• DOI: 10.1016/j.procbio.2013.09.024
• Publication Date: 2013-09-29T10:33:45Z
• Authors: Willian Kopp; Taciane P. da Costa; Sandra C. Pereira; Miguel Jafelicci Jr; Roberto C. Giordano; Rodrigo F.C. Marques; Fernando M. Araúj...; Raquel L.C. Giordano
• Keywords: Cross-linked enzymes aggregates; Superparamagnetic nanoparticles; Penicillin hydrolysis; 540; 01 natural sciences; Magnetic recovery of enzymes; Supramolecular complex; 0104 chemical sciences

Abstract Biomolecules labeled with superparamagnetic nanoparticles can be selectively removed from complex reaction mixtures using an external magnetic field. Amino-functionalized superparamagnetic iron oxide nanoparticles (amino-SPION) were co-aggregated with penicillin G acylase and then cross-linked, generating magnetic cross-linked enzymes aggregates (M-CLEAs) that were quickly and efficiently recovered from the reaction medium by applying an external magnetic field. M-CLEAs and cross-linked enzymes aggregates (CLEAs) prepared under the same reaction conditions were characterized and compared. The best recovered activities were obtained for M-CLEAs prepared using polyethylene glycol 600 as precipitant and the most stable M-CLEA were obtained using tert-butanol. Successive penicillin G hydrolysis reactions were carried out using the same M-CLEA in a 50 mL reactor (3 reaction cycles), after the reactions the derivate was magnetically recovered without loss of activity demonstrating a total magnetic recovery. Line-scan energy dispersive X-ray spectroscopy showed that the amino-SPIONs were homogeneously dispersed within the structure of the M-CLEA.