Analysis of Metabolic Network of Synthetic Escherichia coli Producing Linalool Using Constraint-based Modeling

• DOI: 10.1016/j.procs.2012.09.004
• Publication Date: 2012-10-04T07:31:40Z
• Authors: Iyarest Tawornsam...; Rattana Thanasomboon; Jittrawan Thaiprasit; Dujduan Waraho; Supapon Cheevadha...; Asawin Meechai
• Keywords: Flux Balance Analysis; Metabolic Engineering; Metabolic flux analysis

Linalool is a major volatile compound used frequently as an ingredient of perfumes and fragrances. Typically, it produced by chemical routes that are not eco-friendly. We have previously succeeded to construct synthetic Escherchia coli producing linalool. However, the linalool productivity this strain economically feasible. In work, metabolic network E. was analyzed using constraint-based modeling through flux balance analysis (FBA) single gene deletion enhance production. The results suggest gdh can improve production 14 folds with no growth reduction. distribution reveals fluxes around glyceraldehyde-3-phosphate pyruvate, which intermediates for biosynthesis DXP linalool, markedly increased in gdh-deleted strain.