Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress

• DOI: 10.1016/j.psj.2021.101337
• Publication Date: 2021-06-19T14:46:55Z
• Authors: Rashed A. Alhotan; Ali R. Al Sulaiman; Abdulrahman S. Al...; Alaeldein M. Abud...
• Keywords: Betaine; Occludin; Barrier function

This research was executed to study the impacts of adding betaine (BT) broiler diets on intestinal inflammatory response and barrier integrity under heat stress (HS). At 21 d age, 150 male broilers (Ross 308) were randomly assigned 3 treatment groups: control (CON) group, in which provided standard finisher feed thermoneutral condition (22 ± 1°C); HS group + BT given supplied with 0 1,000 mg/kg BT, respectively, cyclic (33 1°C for 8 h from 08:00 16:00 temperature residual hours). Each replicated ten times 5 per replicate. The showed an elevation (P < 0.05) serum corticosterone (CORT) concentration, D-lactate acid (D-LA) content, diamine oxidase (DAO) activity, mucosal interleukin-1β (IL-1β) level, expression shock protein 70 (HSP70) gene, a reduction interleukin-10 (IL-10) level secretory immunoglobulin A (SIgA) content relative abundance mRNA occludin (OCLN), zonula occludens-1 (ZO-1), claudin-1 (CLDN1), claudin-4 (CLDN4). In contrast, exhibited raise IL-10 SIgA OCLN ZO-1 genes, decline CORT concentration DAO IL-1β HSP70 mRNA. These results indicate that supplemental can ameliorate injury heat-challenged by suppressing responses enhancing function.