Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR

• DOI: 10.1016/j.psj.2023.102730
• Publication Date: 2023-04-20T09:18:22Z
• Authors: Haiqin Li; Zhaofeng Kang; Chunhe Wan; Fanfan Zhang; Meifang Tan; Yanbing Zeng; Chengcheng Wu; Yu Huang; Qi Su; Xiaoquan Guo
• Keywords: TaqMan; Astrovirus

The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes GoAstV, but there is a lack differential diagnosis tools. By analyzing all published genomes this study designed specific PCR primers and Taqman probes recognize different GoAstV. After optimization verification, developed Taqman-based real-time quantitative method that capable diagnosis. established qPCR exhibited detection limitations 100 copies/μL or 10 copies/μL, respectively, for GoAstV genotype 1 2, showed no false positive other common avian viruses. This was then used analyze 72 samples collected from regions Jiangxi, results were verified by genome sequencing phylogenetic analysis. These complex coinfection China, highlighting importance long-term focus on prevalence evolution