Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China

• DOI: 10.1016/j.psj.2023.103271
• Publication Date: 2023-11-16T22:34:38Z
• Authors: Chenyang Yi; Guohong Li; Yinru Mu; Shuyue Cui; Danping Zhang; Qiaoxia Xu; Cheng Liang; Man Wang; Shiwen Zhou; Hongbo Zhou; Ming Zhong; Anding Zhang
• Keywords: Embryonated; Biosecurity; Isolation; In ovo

Multiple outbreaks of avian infectious laryngotracheitis (ILT) in chickens, both domestically and internationally, have been directly correlate to widespread vaccine use affected countries regions. Phylogenetic recombination event analyses demonstrated that virus (ILTV) field strains are progressively evolving toward the chicken embryo-origin (CEO) strain. Even with standardized biosecurity measures effective prevention control strategies implemented on large-scale farms, continuous ILT result significant economic losses poultry industry worldwide. These undoubtedly hinder efforts eradicate ILTV future. In this study, an isolate was successfully obtained by laboratory PCR detection isolation from chickens exhibited dyspnea depression a broiler farm Hubei Province, China. The isolated strain robust propagation chorioallantoic membranes embryonated eggs, but failed establish infection hepatocellular carcinoma (LMH) cells. analysis revealed unique T441P point mutation gJ protein isolate. Animal experiments confirmed virulence strain, as it induced mortality 6-wk-old chickens. This study expands current understanding epidemiology, genetic variations, pathogenicity isolates circulating domestically, contributing elucidate molecular basis development vaccine.