Evaluation and comparison of serological methods for the detection of bovine neosporosis in Argentina

• DOI: 10.1016/j.ram.2015.07.002
• Publication Date: 2015-09-27T12:58:46Z
• Authors: Lucía M. Campero; Lieselotte Minke; Gastón Moré; Magdalena Rambeaud; Diana Bacigalupe; Dadin P. Moore; Yanina Hecker; Carlos M. Campero; Gereon Schares; María C. Venturini
• Keywords: Gold standard (test)

Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. The accurate diagnosis of neosporosis essential for management control measures. aims this study were: i) to evaluate the performance an in-house enzyme-linked immunosorbent assay based on 38kDa native antigen (p38-ELISA) diagnose bovine Argentina using well- characterized local sera panel from experimentally infected naturally exposed ii) compare diagnostic agreement three N. serological tests: p38-ELISA, indirect fluorescence antibody test (IFAT) immunoblotting (IB) same panel. Serum samples testing either positive or negative by IFAT IB were considered "Relative Standards Comparison" (RSC) used p38-ELISA evaluation. Receiver operating characteristics analysis revealed was highly (area under curve= 0.982) according RSC with cut-off index 0.0905. Relative sensitivity specificity 97.8% 99.5%, respectively between almost perfect (k= 0.97). evaluation comparison tests performed definition gold standard decision "majority tests". All displayed high values (greater than 95%); excellent agreement. This describes evaluated locally studied detection anti-N. antibodies Argentina.