Functional hypoxia reduces mitochondrial calcium uptake

• DOI: 10.1016/j.redox.2024.103037
• Publication Date: 2024-01-17T10:02:39Z
• Authors: Chris Donnelly; Timea Komlódi; Cristiane Cecatto; Luiza H.D. Cardoso; Anne-Claire Compa...; Alessandro Matera; Daniele Tavernari; Olivier Campiche; Rosa Chiara Paoli...; Nadège Zanou; Bengt Kayser; Erich Gnaiger; Nicolas Place
• Keywords: Hypoxia; Cellular respiration

Mitochondrial respiration extends beyond ATP generation, with the organelle participating in many cellular and physiological processes. Parallel changes components of mitochondrial electron transfer system render it an appropriate hub for coordinating adaption to oxygen levels. How under functional hypoxia (i.e., when intracellular O2 levels limit respiration) are relayed by impact remodeling after hypoxic exposure remains poorly defined. This is largely due challenges integrating findings controlled defined studies connecting functions isolated mitochondria humans during physical exercise. Here we present experiments conditions mitochondria, myotubes exercising humans. Performing steady-state respirometry found that limitation reduced flow oxidative phosphorylation, lowered membrane potential difference, decreased calcium influx. Similarly, uptake response sarcoplasmic reticulum release contraction. In both human skeletal muscle this blunted adaptive responses upon contractions. Our results suggest regulating a hypoxia.