Time-restricted feeding improves aortic endothelial relaxation by enhancing mitochondrial function and attenuating oxidative stress in aged mice

• DOI: 10.1016/j.redox.2024.103189
• Publication Date: 2024-05-15T15:54:36Z
• Authors: Madison Milan; Jacob Brown; Colleen L. O'Reilly; Matthew P. Bubak; Sharon Negri; Priya Balasubrama...; Arjune S. Dhanekula; Gavin Pharaoh; Zeke Reyff; Cade Ballard; Helen Shi; Andriy Yabluchanskiy; Michael C. Rudolph; Zoltan Ungvari; David J. Marcinek; Benjamin F. Miller; Holly Van Remmen; Stefano Tarantini
• Keywords: Medicine (General); 0303 health sciences; QH301-705.5; Short Communication; Intermittent fasting; Oroboros; 03 medical and health sciences; R5-920; Fluororespirometry; Endothelium; O2K; Biology (General); Mitochondrial dysfunction

Age-related endothelial dysfunction is a pivotal factor in the development of cardiovascular diseases, stemming, at least part, from mitochondrial and consequential increase oxidative stress. These alterations are central to decline vascular health seen with aging, underscoring urgent need for interventions capable restoring function preventing diseases. Dietary interventions, notably time-restricted feeding (TRF), have been identified their anti-aging effects on mitochondria, offering protection against age-associated declines skeletal muscle other organs. Motivated by these findings, our study aimed investigate whether TRF could similarly exert protective vasculature, enhancing reducing To explore this, 12-month-old C57BL/6 mice were placed diet, food access limited 6-h window daily 12 months. For comparison, we included groups young age-matched controls unrestricted feeding. We evaluated impact measuring acetylcholine-induced vasorelaxation aorta. Mitochondrial was assessed using fluororespirometry, reactive oxygen species (ROS) production quantified redox-sensitive dye dihydroethidium. also 4-hydroxynonenal (4-HNE) levels, stable marker lipid peroxidation, aorta ELISA. Our findings demonstrated that aged standard diet exhibited significant impairments aortic relaxation function, associated elevated Remarkably, regimen led substantial improvements parameters, indicating enhanced vasorelaxation, better reduced stress aortas mice. This investigation establishes vital foundation, paving way subsequent clinical research exploring benefits intermittent fasting.